This study assessed the perceived prevalent mentors’ mentoring characteristics on University of Education, Winneba physical education student-interns’ career development in Ghana. A descriptive cross-sectional survey design and census sampling technique guided the development and selection of participants for the study. A self-developed and validated questionnaire with a reliability co-efficient of .74 was used for data collection from 122 PE student-interns. Mean, Standard Deviation and Multiple Linear Regression were used to analyse the Data. The findings indicated that pedagogical content knowledge and satisfaction of mentoring were the least ranked variables for knowledge and psycho-social characteristics respectively. Also, mentors mentoring characteristics accounted for 27% prediction of intern-students career development. It is therefore recommended that Institute of Teacher Education and Continuous Professional and Department of Health, Physical Education, Recreation and Sports UEW should consider mentors’ mentoring characteristics in selecting, training and assigning mentors to student-interns for effective career preparation and development.